It predicts the melting temperature of the given oligonucleotide at a particular salt concentration. The user can provide the salt/Na ion concentration or it would take 0.16 M Na ion concentration as the default concentration.The maximum length of the sequence could be 50 bases.
For Tm Predtiction of longer sequence click here
Sequence Length :
Input File :
Salt Concentration : (Range: 0.001M - 2.5M)
DNA Concentration : (Range: 10-9M - 2.0M)
Predicted Melting Temperature : ± 1.59 °C