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Oligonucleotide Tm Predictor

It predicts the melting temperature of the given oligonucleotide at a particular salt concentration. The user can provide the salt/Na ion concentration or it would take 0.16 M Na ion concentration as the default concentration.The maximum length of the sequence could be 70 bases.

For Tm Prediction of longer sequence click here    For MD Parameter based Tm Predictorclick here     

Input the oligonucleotide sequence


Sequence Length                           :

Input File                                       :


Salt Concentration                         :  (Range: 0.001M - 2.5M)

DNA Concentration                        :   (Range: 10-9M - 2.0M)

Predicted Melting Temperature    :    ± 1.59 °C

                     The dataset used for training in Multiple Regression Analysis is available Here
                     The dataset on which the testing of hypothesis is done is available Here

                         "A phenomenological model for predicting melting temperatures of DNA sequences",
                     Khandelwal G. and Jayaram B., PLoS One 5(8), 2010, e12433.
                     Read Paper

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