The hydrophobicity ratio, ΦH is defined here as the ratio of loss in accessible surface area (ASA) per atom of non-polar atoms to the loss in accessible surface area (ASA) per atom of the polar atoms,
(ΦH) = Loss in ASA per atom of non-polar atoms Loss in ASA per atom of polar atoms
For ~1000 protein dataset, the ratio F H was calculated and a graph between the hydrophobicity ratio and frequency was plotted (Fig.). The hydrophobicity ratio varies from 1.0-1.9 in conformity with expectations. We have selected the range from 1.2-1.6, which lies within the 95% confidence limits as a filter. HOW TO USE TOOL
